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Promega pgem-t-easy cloning kit
Pgem T Easy Cloning Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-t+easy+-ta+cloning+kit/pgem+t+easy/pmc10930869-59-26-29
Average 90 stars, based on 1 article reviews
pgem-t-easy cloning kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Purification:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).

Gel Extraction:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).

Clone Assay:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).

Cloning:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).

Sequencing:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).

Polymerase Chain Reaction:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).

Plasmid Preparation:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).

Synthesized:

Article Title: Pathology, epidemiology, and phylogeny of mussel egg disease due to the microsporidian Steinhausia mytilovum (Field, 1924) in the Mediterranean mussel (Mytilus galloprovincialis).
Article Snippet: In two samples, the microsporidian bands were excised from the gel and the band at 950 bp was cloned with the pGEM-T Easy Vector Cloning Kit (Promega) and then sequenced using the T7 and SP6 plasmid-specific universal primers.

Article Title: Occurrence, Distribution, and Population Structure of Schlumbergera Virus X in Dragon Fruit in Ecuador
Article Snippet: The occurrence of Schlumbergera virus X (SchVX) in commercial dragon fruit fields in three provinces of Ecuador has been identified in this study.. The virus was found in symptomatic and asymptomatic cladodes of the two major species (Hylocereus undatus and H. megalanthus) cultivated in the country.. Symptoms in H. undatus included irregular and ring-shaped chlorotic spots that coalesce into large chlorotic patches along the cladodes, whereas small chlorotic spot symptoms on the cladodes were observed in H. megalanthus.

Article Title: The endoplasmic reticulum kinase PERK interacts with the oxidoreductase ERO1 to metabolically adapt mitochondria.
Article Snippet: The final PCRproductswere cloned using the pGEM-T-easy cloning kit (Promega, Milan, Italy) and sequenced.

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus.
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).

Article Title: Genetic Characterization of Avian Paramyxovirus Isolated from Wild Waterfowl in Korea between 2015 and 2021
Article Snippet: DNA fragments of the expected size were purified using the QIAquick ® Gel Extraction kit (QIAGEN GmbH, Hilden, Germany), and purified DNA was cloned using a pGEM-T-Easy cloning kit (Promega, Madison, WI, USA).

Article Title: CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
Article Snippet: The cloning kit pGEM-T easy vector was purchased from Promega (Madison, WI, USA).


Article Title: Association of different microbes and pathogenic factors in cases of infectious bovine keratoconjunctivitis in cattle from Eastern Kazakhstan
Article Snippet: The synthesized sequences were cloned into the pGEM-T plasmid using the Promega pGEM-T Easy cloning kit. (Promega, Madison, USA).



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